Abstract
Background
The histological responses (HRs) after systemic chemotherapy should be used to determine
the optimal management of patients with peritoneal and liver metastasis from colorectal
cancer (cPM, cLM), in curative intent. We aimed to compare HRs of cPM and cLM in metastatic
mice model after chemotherapy.
Methods
Colon carcinoma CT26-luc cells were transplanted into syngeneic BALB/c mice by intraperitoneal
(leading to cPM), intrasplenic (leading to cLM), or intraperitoneal + intrasplenic
(leading to cPM cLM) injections and follow up using bioluminescence during 21 days.
Bi-chemotherapeutic treatment (5-fluorouracil at D11, D17, and D20, and oxaliplatin
at D13 and D19) was administered. The peritoneal cancer index (PCI) and HRs using
Peritoneal Regression Grading Score (PRGS) and Tumor Regression Grade (TRG) classifications
were analyzed at day 21.
Results
Unlike bioluminescence rate, PCI was reduced after chemotherapy in all treated groups
with cPM comparatively to controls (33 ± 9.5 vs. 19.8 ± 5, p = 0.002 for cPM groups;
37.7 ± 3.6 vs. 25.2 ± 10.8, p = 0.0003 for the cPM + cLM groups). The complete or
major HR rates were higher in all treated groups compared to the non-treated mice
(cPM, 2.29 ± 0.55 vs. 3.56 ± 1.01; cLM, 2.43 ± 1.89 vs. 4.86 ± 0.378; cPM + cLM, 2.73 ± 1.03
and 2.2 ± 0.65 vs. 3.79 ± 0.75 and 4.36 ± 0.43). The complete or major HR rates after
chemotherapy were similar across the metastatic sites in 60% for cPM + cLM group.
Conclusions
The efficacy of chemotherapeutic treatment did not differ between the metastatic sites.
Murine models are suitable in histological analyses to study tumor development and
regression but clinical study will be performed to confirm these results.
Keywords
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Article info
Publication history
Published online: November 17, 2022
Accepted:
November 17,
2022
Received in revised form:
October 12,
2022
Received:
August 5,
2022
Identification
Copyright
© 2022 Published by Elsevier Ltd.
