European Journal of Surgical Oncology
Volume 36, Issue 2 , Pages 160-163, February 2010

Human papillomavirus type 16 E6 gene variations in Chinese population

  • H.B. Cai

      Affiliations

    • Department of Gynecologic Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan 430071, PR China
    • Corresponding Author InformationCorrespondence to: Dr. H.B. Cai, Department of Gynecologic Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430071, PR China. Tel.: +86 27 67812648; fax: +86 27 67813044.
    • ,
  • C.C. Chen

      Affiliations

    • Department of Gynecologic Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan 430071, PR China
    • ,
  • X.H. Ding

      Affiliations

    • Institute of Virology, Wuhan University, Wuhan 430071, PR China

Accepted 31 July 2009.

Abstract 

Objective

To identify genetic variants in the E6 gene of HPV 16 and to examine the association between these variants and cervical cancer development.

Methods

Cervical samples were collected from 72 patients with cervical cancer and 72 age-matched control subjects, all from Hubei Province, China. HPV 16 sequence was detected with E6 gene-specific polymerase chain reaction, and variants were identified by DNA sequencing.

Results

HPV 16 was detected in 81% of the patients and 43% of control subjects, respectively. The rank orders of incidence of HPV 16 prototype and E6 variants were as follows: D25E (62%), the prototype (17%), E113D (9%), L83V (6%) in case subjects; the prototype (71%), D25E (23%), L83V/E113D (7%) in control subjects. The odds ratio of HPV 16 E6 variant was higher than that of prototype (RR=11.73, 95% CI=4.18–32.94, P=0.0001).

Conclusion

The D25 E variant is the most prevalent E6 genomic variant in Hubei, China. The presence of HPV 16 E6 variant in cervical intraepithelial neoplasia (CIN) lesions may serve as a useful predictor of clinical outcome of the disease.

Keywords: Human papillomavirus (HPV), Sequence variation, Cervical carcinoma, Cervical intraepithelial neoplasia

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PII: S0748-7983(09)00411-9

doi:10.1016/j.ejso.2009.07.186

European Journal of Surgical Oncology
Volume 36, Issue 2 , Pages 160-163, February 2010

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