European Journal of Surgical Oncology
Volume 35, Issue 4 , Pages 387-392 , April 2009

Clinical validation of a molecular assay for intra-operative detection of metastases in breast sentinel lymph nodes

  • M.D. Martin Martinez

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • ,
  • I. Veys

      Affiliations

    • Department of Surgery, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • ,
  • S. Majjaj

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • ,
  • L. Lespagnard

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • ,
  • J.C. Schobbens

      Affiliations

    • Department of Surgery, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • ,
  • G. Rouas

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • ,
  • V. Filippov

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • ,
  • D. Noterman

      Affiliations

    • Department of Surgery, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • ,
  • D. Hertens

      Affiliations

    • Department of Surgery, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • ,
  • F. Feoli

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • ,
  • P. Bourgeois

      Affiliations

    • Department of Nuclear Medicine, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • ,
  • V. Durbecq

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • ,
  • D. Larsimont

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
    • Corresponding Author InformationCorresponding author. Translational Research Unit, Head of the Pathology Department, Jules Bordet Institute, Université Libre de Bruxelles, 125 Boulevard de Waterloo, 1000 Brussels, Belgium. Tel.: +32 2 541 31 15; fax: +32 2 541 32 81.
    • ,
  • J.M. Nogaret

      Affiliations

    • Department of Surgery, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium

,Accepted 23 May 2008.

References 

  1. Albertini JL, Lyman GH, Cox C, et al. Lymphatic mapping and sentinel node biopsy in the patients with breast cancer. JAMA. 1996;276:1818–1822
  2. Borgstein PJ, Pijpers R, Comans EF, van Diest PJ, Boom RP, Meijer S. Sentinel lymph node biopsy in breast cancer: guidelines and pitfalls of lymphoscintigraphy and gamma probe detection. J Am Coll Surg. 1986;1998:275–283
  3. Turner RR, Ollila DW, Stern S, Giuliano AE. Optimal histopathologic examination of the sentinel lymph node for breast carcinoma staging. Am J Surg Pathol. 1999;23:263–267
  4. Giuliano AE, Dale PS, Turner RR, Morton DL, Evans SW, Krasne DL. Improved axillary staging of breast cancer with sentinel lymph adenectomy. Ann Surg. 1995;222:394–401
  5. Morton DL. Intraoperative lymphatic mapping and sentinel lymphadenectomy: community standard care or clinical investigation?. Cancer J Sci Am. 1997;3:328–330
  6. Dauway EL, Giuliano R, Pendas S, et al. Lymphatic mapping: a technique providing accurate staging for breast cancer. Breast Cancer. 1999;6:145–154
  7. Veronesi U, Paganelli G, Viale G, et al. Sentinel lymph node biopsy and axillary dissection in breast cancer: results in a large series. J Natl Cancer Inst. 1999;91:368–373
  8. Posther KE, Wilke LG, Giuliano AE. Sentinel lymph node dissection and the current status of American trials on breast lymphatic mapping. Semin Oncol. 2004;31:426–436
  9. White RL, Wilke LG. Update on the NSABP and ACOSOG breast cancer sentinel node trials. Am Surg. 2004;70:420–424
  10. Yared MA, Middleton LP, Smith TL, et al. Recommendations for sentinel lymph node processing in breast cancer. Am J Surg Pathol. 2002;26:377–382
  11. ASCO guideline recommendations for sentinel lymph node biopsy in early stage breast cancer. J Clin Oncol. 2005;23:7703–7720
  12. Schrenk P, Rieger R, Shamiyeh A, Wayand W. Morbidity following sentinel lymph node biopsy versus axillary lymph node dissection for patients with breast carcinoma. Cancer. 2000;88:608–614
  13. Creager AJ, Geisinger KR. Intraoperative evaluation of sentinel lymph nodes for breast carcinoma: current methodologies. Adv Anat Pathol. 2002;94:3016–3022
  14. Creager AJ, Geisinger KR, Shiver SA, et al. Intraoperative evaluation of sentinel lymph nodes for metastatic breast carcinoma by imprint cytology. Mod Pathol. 2002;15:1140–1147
  15. Singletary SE, Connolly JL. Breast cancer staging: working with the sixth edition of the AJCC cancer staging manual. CA Cancer J Clin. 2006;56:37–47
  16. American Joint Committee on Cancer (AJCC) . Cancer staging handbook. 6th ed.. New York: Springer Verlag; 2002;
  17. Groen RS, Oosterhuis AW, Boers JE. Pathologic examination of sentinel lymph nodes in breast cancer by a single haematoxylin–eosin slide versus serial sectioning and immunocytokeratin staining: clinical implications. Breast Cancer Res Treat. 2007;105(1):1–5
  18. Viale G, Bosari S, Mazzarol G, et al. Intraoperative examination of axillary sentinel lymph nodes in breast carcinoma patients. Cancer. 1999;85(11):2433–2438
  19. Treseler PA, Tauchi PS. Pathologic analysis of the sentinel node. Surg Clin North Am. 2000;80(6):1695–1719
  20. Julian T, Blumencranz P, Deck K. A novel intra-operative molecular test for sentinel lymph node metastases in early stage beast cancer patients. J Clin Oncol, in press.
  21. Backus J, Laughlin T, Wang Y, et al. Identification and characterization of optimal gene expression markers for detection of breast cancer metastasis. J Mol Diagn. 2005;7(3):327–336
  22. Blumencranz P, Whitworth PW, Deck K, et al. Sentinel node staging for breast cancer: intraoperative molecular pathology overcomes conventional histologic sampling errors. Am J Surg. 2007;194:426–432
  23. Torrenga H, Rahussen FD, Meijer S, Borgstein PJ, van Diest PJ. Sentinel node investigation in breast cancer: detailed analysis of the yield from step sectioning and immunohistochemistry. J Clin Pathol. 2001;54:550–552
  24. Kurosumi M, Takei H. Significance and problems of histopathological examination and utility of real-time reverse transcriptase-polymerase chain reaction method for the detection of sentinel lymph node metastasis in breast cancer. Breast Cancer. 2007;14:342–349
  25. Viale G, Maiorano E, Pruneri G, et al. Predicting the risk for additional axillary metastases in patients with beast carcinoma and positive sentinel lymph node biopsy. Ann Surg. 2005;11:6528–6535
  26. Viale G, Dell'Orto P, Biasi MO, et al. Comparative evaluation of an extensive histopathologic examination and a real-time reverse-transcription-polymerase chain reaction assay for mammaglobin and cytokeratin 19 on axillary sentinel lymph nodes of breast carcinoma patients. Ann Surg. 2008;247:136–142
  27. Cserni G. What is a positive sentinel lymph node in breast cancer patient? A practical approach. Breast. 2007;16:152–160
  28. Cserni G, Amendoira I, Apostolikas N, et al. Discrepancies in current practice of pathological evaluation of sentinel lymph nodes in breast cancer. Results of a questionnaire based survey by the European Working Group for breast screening pathology. J Clin Pathol. 2004;57:695–701

PII: S0748-7983(08)00193-5

doi: 10.1016/j.ejso.2008.05.008

European Journal of Surgical Oncology
Volume 35, Issue 4 , Pages 387-392 , April 2009

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