Correlation between molecular metastases in sentinel lymph nodes of breast cancer patients and St Gallen risk category

Abstract 

Aims

To evaluate the clinical significance of tumour metastases detected using real-time reverse transcription-PCR (RT–PCR) in sentinel lymph nodes (SLN) of breast cancer patients.

Methods

Sixty-seven patients with T1–T2 primary breast cancer were included in a prospective study. SLN were analysed for the presence of metastatic tumour cells using standard histopathology staining, immunochemistry (IHC) and multimarker real-time RT–PCR assay for mammaglobin (MMG), carcinoembryonic antigen (CEA) and cytokeratin-19 (CK19) mRNA expression. Correlations between molecular metastases and traditional clinicopathological prognostic factors, including St Gallen risk categories were studied.

Results

Of the 67 patients, 15 (22.3%) had one or more pathology-positive SLN. Five (9.6%) pathology-negative SLN were positive by IHC and 19 (36.5%) by RT–PCR. Of note, RT–PCR analysis was also positive in all cases with pathology- or IHC-positive SLN. MMG was the most informative tumour marker in the panel. Molecularly detected metastases were significantly associated with intermediate St Gallen risk category (p=0.023).

Conclusion

Molecular staging of SLN using real-time RT–PCR for early breast cancer could serve as a useful complement to standard clinicopathological risk factors. Studies with long-term follow-up are necessary to define the impact of molecular metastases on disease free survival and overall survival.

Keywords: Breast cancer, Sentinel lymph node, Metastasis, Real-time RT–PCR